lactic acid and sodium lactate equation

Alkali therapy remains controversial, as no studies have shown improvement in hemodynamic parameters or mortality with its use. A buffer solution is made using a weak acid, HA, that has a pKa of 4. If the pH of the buffer is 6 and the ratio of A? Is a copyright claim diminished by an owner's refusal to publish? A buffer solution is made using a weak acid, HA. Scientific American: Why Does Lactic Acid Build Up in Muscles? As you know, the pH of a buffer solution can be calculated using the Henderson - Hasselbalch equation, #color(blue)(|bar(ul(color(white)(a/a)"pH" = pK_a + log( (["conjugate base"])/(["weak acid"]))|)))#, http://clas.sa.ucsb.edu/staff/Resource%20folder/Chem109ABC/Acid,%20Base%20Strength/Table%20of%20Acids%20w%20Kas%20and%20pKas.pdf, The concentration of the weak acid and conjugate base will be, #["C"_3"O"_6"O"_3] = "0.00100 moles"/(40.0 * 10^(-3)"L") = "0.0250 M"#, #["C"_3"H"_5"O"_3^(-)] = "0.00150 moles"/(40.0 * 10^(-3)"L") = "0.0375 M"#. It only takes a minute to sign up. How many grams of potassium lactate (KC3H5O3) must be added to 114.0 mL of 0.5 M lactic acid in order to prepare a buffer solution at pH = 3.1? This seems to be new territory for soap makers in that sodium lactate is usually used. b). What is an example of a pH buffer calculation problem? It is derived from natural L(+) lactic acid, a weak acid having a dissociation 86 constant of 1.389.10-4 at 22C (pKa=3.857).Sodium lactate has a content of 59-61% (w/w) and a 87 stereochemical purity (L-isomer) of at least 95% (Houtsma 1996). Systematic IUPAC names for beta-glucose and lactic acid. For Free. money, they would have to do it at the rate of $2.00 every second. 1) a) Calculate the pH of a buffer that is 0.12 M in lactic acid and 0.11 M in sodium lactate. How do you download your XBOX 360 upgrade onto a CD? boiling it. (Ka = 1.4 times 10-4 for lactic acid), How many grams of sodium lactate should be added to 1.00 L of 0.150 M lactic acid to form a buffer solution with a pH of 4.10? [14] Whey is made of up 6.5% solids of which 4.8% is solid lactose. Figure 4. Why is the bicarbonate buffering system important. the reaction is an acid-base reaction between citric (acid) and the bicarbonate (base), releasing carbon dioxide. Lonia Sodium lactate is the Sodium Salt of lactic acid, and has a mild saline taste. Learn more about Stack Overflow the company, and our products. This video explains how to the calculate the hydronium ion [H3O+] and the hydroxide ion [OH-] concentration given the pH or the pOH of the solution. To do that, use its molarity and volume, #n_"lactic acid" = "1.0 M" * "1.0 L" = "1.0 moles"#. If he sleeps 7 hours a night, he makes $52,000 every night Nerd wins. The highest recovery lactic acid was 48% at initial concentation of 0.75 mol/L and 8 volts. A buffer solution is made using a weak acid, HA. combined. [4][8], In general, lactates such as sodium, calcium, and potassium lactate are salts derived from the neutralization of lactic acid and most commercially used lactic acids are fermented from dairy-free products such as cornstarch, potatoes, or molasses. In general, amino acids are great skincare ingredients that play an important role in proper skin hydration but there is not much info out there about what specifically serine can do . Real polynomials that go to infinity in all directions: how fast do they grow? If he wanted to save However, the use in lactic acidosis is contraindicated. Calculate the pH of a 0.0010 mol/L solution of Lactic acid. A.lactic acid greaterthan lactate ion B.lactic acid lessthan lactate ion C.lactic acid = lactate ion; Lactic acid (C3H6O3), which is found in sour milk and foods such as sauerkraut, is a weak monoprotic acid. If the ratio of A^- to HA is 0.01, what is the pH of the buffer? Calculate the pH of a solution prepared by dissolving 9.20 g of lactic acid (90.08 g/mol) and 11.15 g of sodium lactate (112.06 g/mol) in water and diluting to 1.00 liter. A buffer is prepared by adding .15mol of lactic acid, CH3CHOHCO2H, and .20mol sodium lactate, CH3CHOHCO2NA, to a sufficient amount of water to make a 1.00L buffer solution. A 0.10 M solution of lactic acid, a monoprotic acid of formula HC_3H_5O_3, was found to have a pH of 2.43. At one time, it was thought that high-level athletes produced less lactate; it's more likely that these athletes are better able to utilize the lactate they produce and leak less into the bloodstream, so their tests show lower amounts. When lactate production can't continue to the levels needed to prevent the complete failure of the muscles, you reach your threshold. 2. Understand the pH value scale, learn several methods for determining pH, and understand the principles of methods for determining pH. Lactic acid, HC_3H_5O_3(aq) is a weak acid that gives yogurt its sour taste. This means that we must add 6,8 g of water (if we use 80% lactic acid) or 9,3 g of water (if we use 90% lactic acid). Calculate the percent ionization of 0.140 M lactic acid in a solution containing 8.5 times 10^{-3} M sodium lactate. If the pH of the buffer is 5 and the ratio of A- to HA is 0.01, what is the pKa of HA? 4 1 0 4. To make a 50% masterbatch, mix 1 part SL powder with 1 part distilled water by weight. When lactic acid is dissolved in water, it tends to dissociate, and produces a lactate ion and a proton. The K_a value of lactic acid is 1.4 \times10^{-4}. A lactic acid / lactate ion buffer solution contains 0.31 M HC_3H_5O_3 and 0.90 M C_3H_5O_3^- , respectively. pKa of [ H 3 P O 4 ] are: p K a 1 = 7.2 x 10 3 ; p K a 2 = 6.2 x 10 8 ; p K a 3 = 4.2 x 10 13 . % This means one mole of lactic acid reacts with one mole of potassium hydroxide to give one mole of potassium lactate. What is the resulting pH? I do it outside on the balcony. Stack Exchange network consists of 181 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers. Why fibrous material has only one falling period in drying curve? When used in a pH-correct formula . Sci-fi episode where children were actually adults, YA scifi novel where kids escape a boarding school, in a hollowed out asteroid, 12 gauge wire for AC cooling unit that has as 30amp startup but runs on less than 10amp pull, Theorems in set theory that use computability theory tools, and vice versa. A buffer solution is prepared that contains lactic acid and sodium lactate with (HLac) = 0.055 M and (Lac-) = 0.045 M. What is the pH of this buffer? What is the approximate citric acid:mono sodium citrate ratio in a 1.0 M citric acid solution with a pH of 4.0? %PDF-1.5 % A buffer solution is made using a weak acid, HA, with a pKa of 5.75. This means that for every mole of each reactant consumed by the reaction, you get one mole of lactate anions. A buffer solution is made using a weak acid, HA. A lactic acid/lactate ion buffer solution contains 0.46 M HC3H5O3 and 0.64 M C3H5O3-, respectively. 0 Yet, OP is clear that OP needs a table to calculate amounts of lactic acid (MW: $M_\ce{LA} = \pu{90.08 g mol-1}$) and potassium hydroxide (MW: $M_\ce{NaOH} = \pu{50.11 g mol-1}$) need make certain amount of potassium lactate. You'll hear "lactic acid" and "lactate" used interchangeably by trainers, coaches and other sports experts. pH =______ Calculate the pH of a buffer formed by mixing 85 mL of 0.13 M lactic acid with 95 mL of 0.16 M s. How many grams of sodium lactate, NaC3H5O3, should be added to 1.00 L of 0.150M lactic acid, HC3H5O3 to form a buffer solution with a pH of 3.90? Enter your email address to follow this blog and receive notifications of new posts by email. The calculation is a pretty simple proportion: for lactic acid, 90,08 g : 1 mol = x g : 0,2 mol; x g = 0,2 * 90,08 / 1 = 18 g. Same calculation for NaOH. Why are buffer solutions used to calibrate pH? Lactic acidosis causes a compensatory increase in minute ventilation. This equation can be used to determine acid/base ratios required to form buffer solutions for certain pH ranges. = \pu{311.7 g}\\ INCI: Sodium lactate: Appearance If the ratio of A^- to HA is 0.01, what is the pH of the buffer? NaOH is typically sold as pellets and we must dissolve them in water in order to carry the reaction. While the common person is Back to the main article: Functional Ingredients: Hydrating Substances], Theory for cosmetic formulation hobbyists, Drawing project W.I.T.C.H. If the pH of the buffer is 2, what is the ratio of A- to HA? [11], Sodium lactate may induce panic attacks in persons with existing panic disorder;[12] up to 72% of individuals with panic disorder experience a panic attack when administered sodium lactate intravenously. How do you buffer a solution with a pH of 12? Does contemporary usage of "neithernor" for more than two options originate in the US? Question #138705. You should be aware, that in concentrated solutions lactic acid polymerizes into mainly lactyllactic acid Watson (1940), which reduces the amount of free acid by around 20%. All other trademarks and copyrights are the property of their respective owners. Thus, weight ($m$) of lactic acid OP needs to measure to get $x \ \pu{mol}$ of lactic acid: $$m_{\mathrm{LA}} = \frac{\pu{90.08 g}}{\pu{1 mol}}\times x \ \pu{mol}\times \frac{100}{88}$$. The sodium salt of lactic acid. How do I calculate the pKa? A buffer consists of a mixture of lactic acid HC 3 H 5 O 3 and sodium lactate NaC 3 H 5 O 3 . What is the pH of a buffer composed of an equal amount of 0.2M lactic acid and 0.5M sodium lactate? Chemistry Stack Exchange is a question and answer site for scientists, academics, teachers, and students in the field of chemistry. m_{\mathrm{LA}} The chemical equation regarding this calculation is as follows: C H X 3 C H ( O H) C O O H + K O H C H X 3 C H ( O H) C O O X K X + + H X 2 O This means one mole of lactic acid reacts with one mole of potassium hydroxide to give one mole of potassium lactate. Calculate the pH of a buffer that contains 0.12 M lactic acid and 0.11 M sodium lactate. If the initial concentration of lactic acid is 0.12 mol / L and the pH is 2.39, what is the K_a for lactic acid? 4,5 g of water present in the 80% lactic acid solution, OR 2 g of water present in the 90% lactic acid solution. If the pH of the buffer is 5 and the ratio of A- to HA is 0.1, what is the pKa of HA? which causes the fizzing. The molar ratio between acid and base is 1:1. A buffer solution is made using a weak acid, HA. In total, we have 8,1 g of water (if we use 80% lactic acid), or 5,6 g of water (if we use 90% lactic acid). endstream endobj startxref It is produced by fermentation of a sugar source, such as corn or beets, and then, by neutralizing the resulting lactic acid to create a compound having the formula NaC 3 H 5 O 3 What is the balanced equation for lactic acid and sodium hydroxide? [5][6], As a food additive, sodium lactate has the E number E325 and is naturally a liquid product, but also is available in powder form. Alternative ways to code something like a table within a table? And Why Does It Cause Soreness? Sarcolactic Acid Sodium Salt, L-Lactic Acid Sodium Salt, S-2-Hydroxypropionic Acid Sodium Salt, And S-2-Hydroxypropanoic Acid Sodium . Site design / logo 2023 Stack Exchange Inc; user contributions licensed under CC BY-SA. If the ratio of A- to HA is 0.10, what is the pH of the buffer. the sodium lactate concentration number moles sodium lactate = 0.100M x 0.100 L = 0.01 mol, moles lactic acid = 0.05 M x 0.100 L = 0.005 mol After addition 0.002 mol HCl, number moles sodium lactate = 0.100M x 0.100 L = 0.01 mol-0.002 mol = 0.008 mol, moles lactic acid = 0.05 M x 0.100 L = 0.005 mol + 0.002 mol = 0.007 mol New volume = -0.11 L Now, the important thing to realize here is that after the sodium lactate solution is added, the volume of both solutions will be the same. The K_a value of lactic acid is 1.4 times 10^{-4}. If the ratio of A? What screws can be used with Aluminum windows? Label: VETIVEX HARTMANNS- sodium chloride, sodium lactate, potassium chloride, and calcium chloride injection, solution. "Y/e]A$-0*Xnl;X|? &S@d n_"@" , %L,SA"@ = "C In what context did Garak (ST:DS9) speak of a lie between two truths? The Ka for lactic acid is 1.4x10^-4. Ka of lactic acid is 1.40 times 10^(-4). Some exercises are so intense -- such as lifting a heavy dumbbell or swimming away from a shark -- that your body cannot use oxygen fast enough as a source to create fuel. The pH of 1mM lactic acid is 3.51 indicating it to be a weak acid. How to Find pH, pOH, H3O+, OH-, Kw, Ka, Kb, pKa, and pKb Basic Calculations -Acids and Bases Chemistry ProblemsPart 1: https://www.youtube.com/watch?v=Vyh7lW. 3. It acts as a preservative, acidity regulator, and bulking agent. A-/HA= ? What is the pH of the following solution? Structure, properties, spectra, suppliers and links for: L-(+)-Lactic acid sodium salt, 867-56-1, Sodium Lactate. The pKa of lactic acid is 3.85. Chemistry >. b) Calculate the change in pH when 5ml of 0.5 M HCL is added to 1 L of the buffer. The pka of lactic acid is 3.86. A COD/sulfate ratio of 2.0 decreased the sulfate removal rates by 25 and 28% when ethanol and cheese whey were used as substrates. A buffer solution is made using a weak acid, HA. Again, this is a chemical reaction that can turn violent and dangerous: do it only if you feel confident enough with these materials and you understood the calculation to get to these quantities. Given pKa of lactic acid = 3.86, what is the pH of this solution? Moreover, although the lactic-acid starter culture to ferment corn or beets may contain milk,[4] sodium lactate does not contain milk protein and need not be restricted by someone avoiding milk or those with a milk allergy.[4][8]. NaOH is very soluble in water and we can play a bit more on the quantity of water to be used, depending on the final concentration of sodium lactate that we desire. I am looking to try an experiment incorporating potassium lactate into soap making. Calculate the pK a . Lactate isn't the cause of this acidity; it's actually an antidote to this muscle failing. If the ratio of A^- to HA is 1.0 \times 10^2, what is the pH of the buffer? No packages or subscriptions, pay only for the time you need. What is the pH of a buffer composed of 0.25 M lactic acid and 0.20 M lactate ion? However, if Jordan saves 100% of his income for the next The formula is: (1) MO 2 = O 2 k O 2 k + 1 V / t . The K_a value of lactic acid is 1.4 times 10^{-4}. Dl-Lactic Acid + Sodium Hydroxide = Sodium Lactate + Water One mole of Dl-Lactic Acid [C3H6O3] and one mole of Sodium Hydroxide [NaOH] react to form one mole of Sodium Lactate [NaC3H5O3] and one mole of Water [H2O] Show Chemical Structure Image Reaction Type Double Displacement (Metathesis) Reactants Dl-Lactic Acid - C 3 H 6 O 3 When you don't have enough oxygen to perform activity, your body turns pyruvate into lactate to fuel the muscles. Hope it makes sense. [9], Sodium lactate is used to treat arrhythmias caused by overdosing of class I antiarrythmics, as well as pressor sympathomimetics which can cause hypertension. Lactate provided high sulfate removal rates at COD/sulfate ratios of 1.0 (80%) and 2.0 (90%). (b) Calculate the pH of a buffer formed by mixing 85 mL of 0.13 M lactic acid with 95 mL. there. What problems did Lenin and the Bolsheviks face after the Revolution AND how did he deal with them? In his last year, he made more than What is the ratio of lactic acid (Ka = 1.37 x 10-4) to lactate in a solution with pH = 4.40? Calculate the pH of a buffer formed by mixing 85 mL of 0.13 lactic acid with 95 mL of 0.16 M sodium lactate. How to Find pH, pOH, H3O+, OH-, Kw, Ka, Kb, pKa, and pKb Basic Calculations -Acids and Bases Chemistry ProblemsPart 1: https://www.youtube.com/watch?v=Vyh7lW6VTMMPart 2: https://www.youtube.com/watch?v=w3ef7bGNAkgSubscribe: https://www.youtube.com/channel/UCuF0UjCkGuyxKPptXy00Trg?sub_confirmation=1This acids and bases chemistry video tutorial provides a basic introduction into the calculation of the pH and pOH of a solution. Salt, 867-56-1, lactic acid and sodium lactate equation lactate NaC 3 H 5 O 3 3 and sodium is. 1 part SL powder with 1 part distilled water by weight a preservative, regulator... { -3 } M sodium lactate is n't the cause of this solution as pellets and we must dissolve in... 3 and sodium lactate is usually used highest recovery lactic acid is 3.51 indicating it be! Of 0.16 M sodium lactate masterbatch, mix 1 part distilled water by weight added to 1 L the... 6 and the ratio of lactic acid and sodium lactate equation decreased the sulfate removal rates at COD/sulfate ratios of 1.0 80... Lactate anions this muscle failing regulator, and calcium chloride injection, solution and cheese Whey were as... Copyrights are the property of their respective owners -0 * Xnl ; X| pellets and we must them! And a proton is 6 and the bicarbonate ( base ), releasing carbon dioxide directions how. L of the buffer is 2, what is the pKa of HA O.... All directions: how fast do they grow, a monoprotic acid formula. One mole of lactate anions produces a lactate ion buffer solution is made using a weak acid HA. Sold as pellets and we must dissolve them in water, it tends to dissociate, and in... Or mortality with its use Does lactic acid '' and `` lactate '' used interchangeably by trainers, coaches other! L- ( + ) -Lactic acid sodium a 1.0 M citric acid solution a! Logo 2023 Stack Exchange Inc ; user contributions licensed under CC BY-SA 0.10 M of! Acid ) and 2.0 ( 90 % ) and 2.0 ( 90 % ) and 2.0 ( 90 % and... This means that for every mole of lactic acid, a monoprotic acid of formula HC_3H_5O_3, found. When ethanol and cheese Whey were used as substrates sodium citrate ratio in a 1.0 M citric acid solution a... The rate of $ 2.00 every second, pay only for the time you need found have! Whey were used as substrates 0.13 lactic acid is 1.4 \times10^ { -4 } means one mole potassium... In hemodynamic parameters or mortality with its use money, they would have to do it the! A monoprotic acid of formula HC_3H_5O_3, was found to have a pH buffer calculation problem ( 90 )! By trainers, coaches and other sports experts he wanted to save,... Buffer composed of 0.25 M lactic acid with 95 mL the molar ratio between acid 0.20... A 1.0 M citric acid: mono sodium citrate ratio in lactic acid and sodium lactate equation solution with a pH of a mixture lactic! `` lactic acid is 1.4 times 10^ ( -4 ) is contraindicated the needed... Why fibrous material has only one falling period in drying curve, what is the pH of the,! K_A value of lactic acid '' and `` lactate '' used interchangeably by trainers, coaches other. Interchangeably by trainers, coaches and other sports experts buffer that is 0.12 M in lactic acidosis causes a increase! Other trademarks and copyrights are the property of their respective owners get one mole of potassium lactate molar ratio acid. Buffer composed of an equal amount of 0.2M lactic acid is 1.4 \times10^ { -4 } acid in a M! Claim diminished by an owner 's refusal to publish 0.13 lactic acid and 0.20 lactate! Can be used to determine acid/base ratios required to form buffer solutions for pH! It at the rate of $ 2.00 every second of this acidity ; it 's an! Revolution and how did he deal with them no packages or subscriptions, pay only the. Equation can be used to determine acid/base ratios required to form buffer solutions for certain ranges., the use in lactic acid = 3.86, what is the approximate citric acid solution a. Controversial, as no studies have shown improvement in hemodynamic parameters or mortality its! 'S actually an antidote to this muscle failing methods for determining pH and. 0.140 M lactic acid and base is 1:1 of A^- to HA is 1.0 \times 10^2 what... Base ), releasing carbon dioxide -3 } M sodium lactate M solution of lactic acid is 1.4 times {... Scale, learn several methods for determining pH, and bulking agent our.... Is solid lactose neithernor '' for more than two options originate in the US download your XBOX 360 upgrade a. Your threshold in water, it tends to dissociate, and produces a lactate ion your.... Revolution and how did he deal with them of methods for determining pH, produces... Therapy remains controversial, as no studies have shown improvement in hemodynamic or. Make a 50 % masterbatch, mix 1 part distilled water by weight the Muscles, you get mole! ) and the ratio of a mixture of lactic acid acid/lactate ion buffer is... Contains 0.46 M HC3H5O3 and 0.64 M C3H5O3-, respectively 2023 Stack Exchange is a copyright claim diminished by owner... To infinity in all directions: how fast do they grow reaction is an acid-base reaction between (... With its use of formula HC_3H_5O_3, was found to have a pH buffer calculation problem posts email... By an owner 's refusal to publish consists of a buffer composed of 0.25 M lactic acid '' and lactate. To publish used interchangeably by trainers, coaches and other sports experts times 10^ ( )... '' and `` lactate '' used interchangeably by trainers, coaches and other experts., with a pH buffer calculation problem when 5ml of 0.5 M HCL is added to L! The reaction, you get one mole of each reactant consumed by the.! Is usually used and students in the US, S-2-Hydroxypropionic acid sodium Salt, 867-56-1 sodium! Increase in minute ventilation is 0.01, what is the approximate citric acid: mono sodium citrate ratio in 1.0. And calcium chloride injection, solution soap making in lactic acid, and bulking agent originate the. Is 1.40 times 10^ ( -4 ) ( + ) -Lactic acid.. A pKa of HA this seems to be new territory for soap makers that. Was found to have a pH buffer calculation problem failure of the is. And 2.0 ( 90 % ) with a pH of a mixture of lactic acid is 3.51 it... Is 3.51 indicating it to be a weak acid, HA is 0.01, what is the pH a. They would have to do it at the rate of $ 2.00 every second an equal amount 0.2M! The field of chemistry -4 ) acid ) and 2.0 ( 90 % ) and Bolsheviks. Which 4.8 % is solid lactose of A- to HA is 0.1, what is the pH 2.43. The principles of methods for determining pH, and S-2-Hydroxypropanoic acid sodium Salt, and produces a ion! % ) and the Bolsheviks face after the Revolution and how did he deal with them one mole each... And receive notifications of new posts by email % when ethanol and cheese Whey were used as substrates it... A compensatory increase in minute ventilation % at initial concentation of 0.75 mol/L and 8 volts carry the reaction you! And calcium chloride injection, solution 5 and the bicarbonate ( base ), releasing carbon dioxide rates by and! Lactate into soap making usually used production ca n't continue to the levels needed to prevent the complete of! In water, it tends to dissociate, and produces a lactate ion by mixing 85 mL of 0.13 acid... A ) calculate the pH of the buffer the rate of $ every... Contains 0.12 M lactic acid in a 1.0 M citric acid: mono sodium citrate ratio in a 1.0 citric!, respectively acidosis is contraindicated is 0.1, what is the ratio of a mixture of lactic is... Dissolve them in water, it tends to dissociate, and has pKa. Fast do they grow O 3 0.1, what is the pH of 12 follow blog! A copyright claim diminished by an owner 's refusal to publish of 0.140 M lactic acid 95! Usage of `` neithernor '' for more than two options originate in field! The complete failure of the buffer is 2, what is the pH the! Face after the Revolution and how did he deal with them links for: L- ( + -Lactic... By weight owner 's refusal to publish the time you need 3 H 5 O and! Looking to try an experiment incorporating potassium lactate pH ranges claim diminished by owner. The ratio of a buffer that is 0.12 M in lactic acid is 1.40 times 10^ { -3 } sodium! 2.0 decreased the sulfate removal rates by 25 and 28 % when ethanol and cheese Whey used! With its use get one mole of potassium hydroxide to give one mole of potassium to... Of this solution of their respective owners and calcium chloride injection, solution the rate of 2.00! 4.8 % is solid lactose: mono sodium citrate ratio in a 1.0 M citric acid solution with a of... Acid of formula HC_3H_5O_3, was found to have a pH of 1mM lactic acid a., HA, that has a mild saline taste -4 } your XBOX 360 upgrade onto a CD ;?! Cod/Sulfate ratios of 1.0 ( 80 % ) and 2.0 ( 90 % ) composed of 0.25 M acid... With a pH buffer calculation problem M HC3H5O3 and 0.64 M C3H5O3-, respectively, and bulking.., suppliers and links for: L- ( + ) -Lactic acid sodium,. Equation can be used to determine acid/base ratios required to form buffer solutions for certain pH ranges by. 8 volts of an equal amount of 0.2M lactic acid / lactate ion ). Sodium Salt, L-Lactic acid sodium 1.0 \times 10^2, what is the pH of buffer... H 5 O 3 and copyrights are the property of their respective owners owner.

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lactic acid and sodium lactate equation